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Accumulation of selected mRNAs in Rvb2p-depleted cells. GAL::rvb2 test cells were grown in medium containing 2% galactose, washed, and shifted to medium containing 2% glucose. Total RNA was extracted from cells collected before (left lane) and after (other lanes) the medium shift, fractionated on a 1.5% agarose-formaldehyde gel, and incubated with appropriate radiolabeled antisense probes. Incubation times in glucose are indicated above each lane. 18S rRNA served as a loading control.
