FIG. 5.

Nuclear location of Rvb2p and mouse p50. (A) Distribution of Rvb2-GFP in yeast. Yeast cells (YWD312) each harboring a plasmid-borne copy of Rvb2-GFP (pWD235WGFP) were grown to log phase in selective medium. The cells were incubated with a Cy3-labeled oligonucleotide probe specific for U3, and the subnuclear localizations of p50-GFP and U3 were examined by fluorescence microscopy (top row). Nuclear DNA was visualized with DAPI (bottom left), and cell morphology was visualized with DIC (bottom right). (B) Localization of the mouse homolog of Rvb2 in COS-7 cells. Cells were transformed by the calcium-phosphate procedure (58) with a plasmid (pTK147) carrying mouse Rvb2 fused to GFP. Localization of Rvb2-GFP was observed by fluorescence microscopy (left), and nucleoli were visualized with phase contrast (right). Each field is 30 by 30 μm; arrows, nucleoli.