FIG. 6.

Loss of Rvb2p causes delocalization of C/D and H/ACA snoRNP proteins. GAL::rvb2 test strains containing chromosomally encoded Nop1p (YWD313) or a plasmid encoding a Gar1p-GFP fusion were grown in minimal selective galactose medium and shifted to glucose medium for 10 h to halt production of RVB2 mRNA (see Materials and Methods). Nop1p was detected with a primary monoclonal antibody, followed by a fluorescein isothiocyanate-coupled secondary antibody and observed by immunofluorescence microscopy. Gar1p-GFP was observed by direct fluorescence microscopy. (A) Localization of Gar1p in wild-type cells (upper left) and cells depleted of Rvb2p (lower left). (B) Localization of Nop1p in wild-type cells (upper left) and Rvb2p-depleted cells (lower left). The nucleus was visualized with a DNA stain (DAPI; middle). A merged view is also shown (right).