FIG. 8.

Accumulation of snoRNA is impaired in cells harboring rvb2 ts mutations. Results of Northern blot analysis are shown for box H/ACA snoRNAs (A), box C/D snoRNAs (B), splicing snRNAs and RNase P (C), and EF-1β, ASC1, TEF4, RPS5, and RPL32 mRNAs (D). Panels A to C include total RNA from wild-type cells (WT) and cells with the rvb2 K81A allele (K81A) or G80A allele (G80A). Times of incubation at 37°C are indicated above each lane. Time zero identifies samples harvested prior to the temperature shift. Northern blots in panel D are of RNA extracted from cells grown at 30°C.