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. 2022 Dec 19;15(3):e16244. doi: 10.15252/emmm.202216244

Figure 2. Microbiome analysis of 3m mdx mice.

Figure 2

  1. Observed number of enriched ASVs in 3m C57Bl (n = 6) (maximum: 666; median: 583.67; minimum: 475) and 3m mdx (n = 8) (maximum: 522; median: 465.625; minimum: 404). Data are presented as the exact number of ASVs (*P < 0.05; Student's t‐test).
  2. PCA of beta‐diversity of 3m C57Bl (n = 6) and 3m mdx (n = 7) as measured by Unweighted UniFrac distance and Bray–Curtis dissimilarity.
  3. Mean relative abundance at genus level among groups. All genera with relative abundance < 0.1% are reported together and labeled as “others.”
  4. Volcano plots of 3m C57Bl (n = 6) and 3m mdx (n = 7) showing the significantly enriched bacterial amplicon sequence variants (ASVs) (with P < 0.05) by the DEseq2 analysis. The names of the significantly enriched bacterial ASVs classified to the genus level and P < 0.005 are reported. All P‐values were false discovery rate–corrected.
  5. Random forest analysis. The top 20 bacterial genera with the highest discriminatory power sorted by mean decrease GINI value are showed.
  6. Relative abundance of different genus in 3m C57Bl (n = 6) and 3m mdx (n = 7). Prevotella: 3m C57Bl: maximum: 0.28438734; median: 0.162312199; minimum: 0. 3m mdx: maximum: 4.650449086; median: 1.928565583; minimum: 0.308938765. Alistipes: 3m C57Bl: maximum: 2.381488226; median: 1.622780995; minimum: 0.52990159. 3m mdx: maximum: 24.79693926; median: 11.74146327; minimum: 5.71434417. Parasutterella: 3m C57Bl: maximum: 1.491499069; median: 0.923747366; minimum: 0.390776848. 3m mdx: maximum: 0.114573317; median: 0.036571394; minimum: 0.002045952. Rikenella: 3m C57Bl: maximum: 0.202549256; median: 0.124121093; minimum: 0.067516419. 3m mdx: maximum: 0; median: 0; minimum: 0. *P < 0.05; Student's t‐test.
  7. Multidimensional scaling analysis of small intestinal metabolomic profiles from of 3m C57Bl (n = 4) and 3m mdx (n = 3) mice calculated by samples' distance similarities (Bray‐Curtis) with the most discriminatory metabolites (top variable importance in projection score) identified.
  8. Concentration of the significantly different metabolites isolated from the small intestinal content of 3m C57Bl (n = 4) and 3m mdx (n = 3) mice. *P < 0.05, **P < 0.01 and ***P < 0.001; Wilcoxon rank‐sum test.

Source data are available online for this figure.