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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: FASEB J. 2022 May;36(5):e22328. doi: 10.1096/fj.202200251R

Figure 3. IL-4 administration induces apoptosis via P53 and caspase activation.

Figure 3.

(A)​ Expression of Trp53 assessed by qPCR analysis in the bone marrow and spleen of AML mice. Data were normalized to 18S rRNA expression. (n=6~7). (B)(C) Western blot showing expression of P53 in cells isolated from bone marrow (B) and spleen (C) of AML mice. Densitometry was done by normalizing to vehicle control and relative to β-actin. (n=4~6). (D)(E) Expression of TP53 (D) and CDKN1A (E) assessed by qPCR analysis in patient AML cells treated with vehicle or IL-4 (n=5); each line represents a single patient sample treated with vehicle or IL-4. Data were normalized to 18S rRNA expression. (F)(G) Western blot showing expression of CASPASE-3 in cells isolated from bone marrow (F) and spleen (G) of AML mice. Densitometry was done by normalizing to vehicle control and relative to β-actin; n=4~6. (H)(I) Western blot showing expression of cleaved CASPASE-3 in cells isolated from bone marrow (H) and spleen (I) of AML mice. Densitometry was done by normalizing to vehicle control and relative to total Caspase-3; n=4~6. (A-C, F-I) Data shown are mean ± SEM per group; paired two-tailed Student t test was applied to D and E; and unpaired two-tailed Student t test was utilized for other panels; *, P < 0.05; **, P < 0.01.