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. 2001 Nov;21(22):7775–7786. doi: 10.1128/MCB.21.22.7775-7786.2001

FIG. 5.

FIG. 5

Expression and cell viability of slow-growth hTERT mutants. (A) Anti-FLAG Western blot of slow-growth (+14 and +110) and nonessential (+212 and +422) hTERT mutants transiently expressed in 293T cells. Equal loading is shown by the anti-actin blot. (B) Viability of slow-growth and immortal HA5 cells was determined by flow cytometry of annexin V and propidium iodide double-stained cells. The percentages shown are averages for three independent experiments.