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. 2023 Feb 24;19(2):e1011202. doi: 10.1371/journal.ppat.1011202

Fig 2. Expression of HBZ correlates with MyoF expression.

Fig 2

(A) Deletion of HBZ in ST1 and KK1 ATL cells reduces MYOF expression. The graph was generated from published microarray data (GEO accession number GSE94409 [56]) and shows the percent reduction in MYOF transcript levels seven days after inducing CRISPR/Cas9-mediated knockout of HBZ in the ATL cell lines, ST1 and KK1, using two different guide RNAs (sgHBZ #1 and #2) compared to the guide RNA control. Data were obtained using GEO2R with calculations based on averaged values from the three array features probing for different regions of the MyoF transcript; ** p<0.01, *** p<0.001. (B) Relative MYOF mRNA levels in HTLV-1-immortalized human T-cell clones recently established from peripheral blood lymphocytes (PBL). The graph shows qRT-PCR results as follows: C3, value from one RNA extraction; ΔHBZ clones, values averaged from two extractions; all others, values averaged from three extractions. Values were normalized to those for the resting CD4+ T-cells (set to 1). Dark gray bars show clones derived from different PBL donors immortalized with HTLV-1-wt (CJ2, CJ4, C13, C15). Hatched bars show clones derived from different PBL donors immortalized with HTLV-1-ΔHBZ (C9-1 and C13-1). Black bars show clones immortalized with either HTLV-1-wt or HTLV-1-ΔHBZ derived from the same PBL donor (C2, C3, C6, C2-11 and C12). Light gray bars show a subset of cells also analyzed in Fig 1C for comparison. (C) Relative tax and hbz mRNA levels in HTLV-1-immortalized human T-cell clones recently established from PBL. The graph shows qRT-PCR results averaged from the same set of RNA specimens analyzed in (B) in which values were normalized to those for the SLB-1 T-cells (set to 1).