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. 2023 Feb 24;19(2):e1011202. doi: 10.1371/journal.ppat.1011202

Fig 9. MyoF increases levels of total extracellular and virion-associated SU (gp46).

Fig 9

(A) Knockdown of MyoF expression reduces overall levels of SU in the culture medium of HTLV-1 cells. TCA-precipitated proteins from culture media of SLB-1 cells stably transfected with shRNA targeting GFP or MYOF mRNA were divided into equal fractions and analyzed by Western blot using an antibody against SU/Pr (upper panel) and, separately, by Coomassie blue-staining following SDS-PAGE (lower panel). Whole cell extract from SLB-1 cells served as a positive control. (B) Knockdown of MyoF expression reduces levels of SU in cell-free HTLV-1 virions. Culture media from SLB-1 cells stably expressing an shRNA targeting GFP or MYOF mRNA were filtered, ultracentrifuged and analyzed by Western blot using antibodies against SU/gp46 and Gag p19; * denotes a nonspecific band from the serum. (C) Knockdown or inhibition of MyoF does not affect levels of virus released from the cells. Clarified culture media from SLB-1 cells stably expressing an shRNA targeting GFP or MYOF mRNA and SLB-1 and ATL-2 cells treated with DMSO or 1 μM WJ460 for 24 h were analyzed by ELISA to quantify levels of Gag p19. The graph shows values averaged from three replicates.