Fig. 1.

Characterization of plasma extracellular vesicle and protein-enriched fractions from SIV-uninfected and infected nonhuman primate models.

(a) Particle concentrations of EV (left) and protein (right) enriched fractions of uninfected, untreated, and rebound animals were measured by HSFCM. Particle concentration for each group was normalized by plasma input (per 1 ml). (b) Size distributions of EV (left) and protein (right) fractions were measured by HSFCM and calculated as particles in a specific size bin versus total detected particles in each sample (percentage). (c) Viral load (GAG RNA qPCR) as copy number per ml plasma for unfractionated plasma, EVs, and proteins. Data are mean ± SD. ns, no significant difference (P > 0.05), ^∗P ≤ 0.05, ^∗∗∗P ≤ 0.001 by two-tailed Welch's t-test.