Fig. 3.

EV miRNA validation in pigtailed macaques.

(a) qPCR validation for miR-342–3p, miR-181a, and miR-29a in pigtailed macaques (n = 12) at different infection phases. Delta Cq values were normalized to the spike in cel-miR-39 control. Data are mean ± SD. ^∗P ≤ 0.05, ^∗∗P ≤ 0.01, ^∗∗∗P ≤ 0.001, ^∗∗∗∗P ≤ 0.0001 by two-tailed Welch's t-test. (b) Receiver operating characteristic (ROC) curves for the levels of three individual EV miRNAs and a combined three-miRNA panel to differentiate preinfection from acute infection (7 and 14 dpi), latency, and rebound.