FIG. 8.

Small RNA fragments generated by Drosophila S2 and mammalian cells. Cytoplasmic extracts (50 μg) from various mammalian cells were incubated with 30 nmol of radiolabeled dsRNA for 1 h at 30°C for S2 cells or 37°C for mammalian cells. After the reaction, samples were treated with proteinase K–0.5% SDS. The size of dsRNA was examined on a 12% denaturing acrylamide gel. Probe indicates the radiolabeled dsRNA made by in vitro transcription. Lane M, 10-bp markers.