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. 2023 Mar 8;9(10):eade8582. doi: 10.1126/sciadv.ade8582

Fig. 5. Expression of cAMP/CRP during cycles of intermittent daily exposure to AMP.

Fig. 5.

In (A) to (D) and (J), E. coli K12 was cultured in LB medium with or without AMP (100 μg/ml) for 4.5 hours at 37°C followed by 16 hours in LB medium with or without AMP (0.625 μg/ml) per day, respectively, for the indicated number of cycles/days. (A) Expression of crp with increasing number of cycles exposed to AMP (n = 4). (B) Quantitation of cAMP with increasing number of cycles exposed to AMP (n = 4). (D) Growth/viability and MIC of the indicated ∆crp cells after the indicated number of cycles exposed to AMP (n = 4). (E) Expression of ptsL, ptsH, crr, and ptsG with and without 4 mM glucose in wild type and ∆crp (n = 3). (F to I) qRT-PCR for the expression of the indicated genes in wild type and ∆crp E. coli K12 (n = 3). (J) ROS of ∆crp in the presence or absence of AMP (n = 4). (K) Expression of the indicated genes in wild type and ∆crp (n = 3). (L) SOD activity in wild type and ∆crp (n = 3). Results are displayed as means ± SEM, and statistically significant differences are identified by Kruskal-Wallis followed by Dunn’s multiple comparison post hoc test for all the tests unless otherwise indicated. *P < 0.05 and **P < 0.01.