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. 2023 Mar 8;9(10):eade8582. doi: 10.1126/sciadv.ade8582

Fig. 7. Expression of DNA repair genes and ampC in wild type and ∆crp E. coli K12 and during cyclic intermittent exposure to AMP.

Fig. 7.

(A, C, and E) qRT-PCR for the expression of the indicated genes in wild type and ∆crp. (A) MMR, (C) base excision repair system, and (E) ampC and its related genes (n = 3). (B, D, and F to H) E. coli K12 cells were cultured as described in the legend of Fig. 1 (n = 4). qRT-PCR was used to quantify the expression of the indicated genes during cyclic exposure to AMP, as indicated. (B) MMR, (D) base excision repair system, and (F to H) ompR, bola, and ampC, respectively. (I) Activity of AmpC in the evolutionary cycle was measured using susceptibility test disks. Threshold value = 18 (n = 4). Results are displayed as means ± SEM, and statistically significant differences are identified by Kruskal-Wallis followed by Dunn’s multiple comparison post hoc test for all the tests unless otherwise indicated. *P < 0.05 and **P < 0.01.