Table 3. DCE-derived parameters from 21 ROI (matching those analyzed by means of MR-DWI) in 5 KO and 6 WT littermate mice, along with associated nonparametric pair-wise statistics using a two-tailed Wilcoxon signed-rank test and median ± standard deviation (SD) values strain-wise.
Legend: Mean AUC – mean from areas under the DCE curves along with associated p-statistical values (p-stat) from nonparametric Mann–Whitney U-test ROI-wise; Duration – duration of significantly different from the baseline signal enhancement, mimicking the parenchymal tracer accumulation; Aqp4(+/+) – wildtype control; Aqp4(-/-) – AQP4 KO mice; Δ – WT-KO difference; rel. Δ – ((WT-KO)/WT)×100% relative difference (negative ‘-‘ sign means the shorter duration of interstitial tracer accumulation in KO mice); For ROI abbreviations, see caption Figures 2 and 3, or Table 2. # –Two-way Anova with Bonferroni's post-hoc. ¶ – mean of standard deviations ROI wise. * – p<0.05, ** – p<0.01.
| #WT vs. KO time-series different? | Mean AUC [a.u.] | Arrival time [min] | Time- to-peak [min] | Peak intensity [a.u.] | Interstitial tracer accumulation time [min] | Duration [min] | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ROI | Aqp4 (+/+) | Aqp4 (-/-) | p-stat | Aqp4 (+/+) | Aqp4 (-/-) | Δ | Aqp4 (+/+) | Aqp4 (-/-) | Δ | Aqp4 (+/+) | Aqp4 (-/-) | rel. Δ [%] | Aqp4(+/+) | Aqp4(-/-) | Aqp4 (+/+) | Aqp4 (-/-) | rel. Δ [%] | ||||
| Start | End | Start | End | ||||||||||||||||||
| Cortical | OLF | NS | 7207 | 5619 | NS | 6 | 6 | 0 | 61 | 52 | 9 | 106 | 79 | 25.5 | 43 | 80 | 39 | 70 | 38 | 32 | –15.8 |
| CA/RSP | ** | 1662 | 616 | ** | 7 | 7 | 0 | 89 | 90 | -1 | 27 | 12 | 55.6 | 59 | 90 | 66 | 90 | 32 | 25 | –21.9 | |
| VIS | ** | 1699 | 529 | ** | 7 | 8 | -1 | 89 | 90 | -1 | 33 | 9 | 72.7 | 54 | 90 | 73 | 90 | 37 | 18 | –51.4 | |
| SS | NS | 537 | 438 | NS | 6 | 7 | -1 | 20 | 16 | 4 | 8 | 7 | 12.5 | 12 | 32 | 11 | 25 | 21 | 15 | –28.6 | |
| AUD | NS | 454 | 340 | NS | 7 | 7 | 0 | 89 | 89 | 0 | 10 | 5 | 50.0 | 67 | 90 | N/A | N/A | 24 | N/A | <-100 | |
| HIP | ** | 3258 | 1622 | * | 5.5 | 7.5 | -2 | 63 | 81 | –18 | 46 | 22 | 52.2 | 45 | 90 | 74 | 90 | 46 | 17 | –63.0 | |
| PERI | P=0.052 | 3513 | 2659 | NS | 4 | 5 | -1 | 83 | 90 | -7 | 59 | 44 | 25.4 | 53 | 90 | 57 | 90 | 38 | 34 | –10.5 | |
| Brain stem | TH | * | 3471 | 2557 | 0.052 | 2–3 | 2–3 | 0 | 82 | 90 | -8 | 61 | 45 | 26.2 | 53 | 90 | 58 | 90 | 38 | 33 | –13.2 |
| HAB | NS | 2379 | 1992 | NS | 9 | 11 | -2 | 89 | 90 | -1 | 45 | 36 | 20.0 | 54 | 90 | 58 | 90 | 37 | 33 | –10.8 | |
| HY | NS | 7908 | 6766 | NS | 4 | 4 | 0 | 39 | 34 | 5 | 123 | 105 | 14.6 | 23 | 59 | 21 | 54 | 37 | 34 | –8.1 | |
| MB | * | 6125 | 4013 | * | 3 | 4 | -1 | 66 | 83 | –17 | 87 | 56 | 35.6 | 42 | 84 | 53 | 90 | 43 | 38 | –11.6 | |
| PAG | NS | 25740 | 22474 | NS | 8 | 10 | -2 | 43 | 38 | 5 | 350 | 311 | 11.1 | 35 | 75 | 31 | 61 | 41 | 31 | –24.4 | |
| HB | NS | 9291 | 8124 | NS | 2–3 | 2–3 | 0 | 42 | 38 | 4 | 133 | 114 | 14.2 | 28 | 66 | 27 | 61 | 39 | 35 | –10.3 | |
| Nuclei / tracts | CP | NS | 1736 | 1167 | NS | 4 | 8 | -4 | 89 | 90 | -1 | 39 | 25 | 35.9 | 54 | 90 | 58 | 90 | 37 | 33 | –10.8 |
| WM | ** | 6953 | 5445 | ** | 4.5 | 6.5 | -2 | 51 | 53 | -2 | 105 | 80 | 23.8 | 37 | 73 | 38 | 69 | 37 | 32 | –13.5 | |
| CSF space | CM | NS | 23276 | 19903 | NS | 1 | 1 | 0 | 24 | 24 | 0 | 397 | 339 | 14.6 | 15 | 50 | 16 | 47 | 36 | 32 | –11.1 |
| PCS | NS | 15163 | 12498 | NS | 2–3 | 2–3 | 0 | 41 | 31 | 10 | 224 | 187 | 16.5 | 23 | 59 | 20 | 50 | 37 | 31 | –16.2 | |
| 3V | NS | 12228 | 10441 | NS | 5 | 6 | -1 | 45 | 46 | -1 | 180 | 151 | 16.1 | 34 | 76 | 33 | 61 | 43 | 29 | –32.6 | |
| LV | NS | 8288 | 6555 | NS | 6 | 11 | -5 | 90 | 90 | 0 | 157 | 123 | 21.7 | 54 | 90 | 58 | 90 | 37 | 33 | –10.8 | |
| Caudal | SSS | * | 5832 | 1430 | ** | 1 | 3 | -2 | 64 | 88 | –24 | 77 | 22 | 71.4 | 38 | 90 | 75 | 90 | 53 | 16 | –69.8 |
| CB | NS | 7369 | 6151 | NS | 6 | 7 | -1 | 47 | 59 | –12 | 97 | 80 | 17.5 | 29 | 75 | 31 | 67 | 47 | 37 | –21.3 | |
| MEDIAN ±SD | 6125±1653¶ | 4013±1306¶ | 5.0±2.2 | 6.5±2.8 | 63±23 | 81±27 | 56 | 87 | 24±19 | 37±7 | 32±7 | ||||||||||
|
KO vs. WT difference
(Wilcoxon signed-rank test) |
WT >KO, p<0.0001 | WT <KO, p<0.01 | WT ~KO, p=0.2971 | WT >KO, p<0.0001 | WT >KO, P<0.0001 | ||||||||||||||||