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. Author manuscript; available in PMC: 2023 Jul 16.
Published in final edited form as: Nat Metab. 2023 Jan 16;5(1):165–181. doi: 10.1038/s42255-022-00722-6

Extended Data Fig. 3 ∣. Aster-mediated cholesterol transport determines the size of the accessible PM cholesterol pool.

Extended Data Fig. 3 ∣

a, PM total lipids from mouse liver after 4- and 16-h fasting as determined by mass spec (n = 3/3). b, Immunoblot analysis of ALOD4 binding in F/F control and L-A/C KO primary hepatocytes after treatment with vehicle, nSmase (100 mU/ml) and GW4869 (10 μM) for 1-h. Actin was used as a loading control. c, Expression levels of the indicated genes in primary hepatocyte that had been cultured in Maintenance medium and Ro 48-8071 (1μM) overnight before being treated with or without oleic acid (30μM) for 6-h (n = 3). d, Immunoblot analysis of ALOD4 binding in primary hepatocytes after treatment with vehicle or indicated concentration of glucagon, acetoacetate (Ac-Ac), beta-hydroxybutyrate (BHB) or MβCD-cholesterol (35 μM) for 1-h. Calnexin was used as a loading control. e, Immunoblot analysis of ALOD4 binding in primary hepatocytes after treatment with vehicle or insulin (300 nM), glucose (50 mM) and both for 1-h. Calnexin was used as a loading control. All data are presented as mean ± SEM.