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. 2023 Feb 23;14:1081572. doi: 10.3389/fimmu.2023.1081572

Figure 4.

Figure 4

Effect of AFP on the migration of macrophages and apoptosis of HCC cells. M0 macrophages were infected with AFP-expressing negative control lentivirus vectors(M0-NC) or AFP-expressing lentivirus vectors(M0-AFP) for 48 h and then treated with LPS+IFN-γ for 24 h to induce M0 macrophage polarize into the M1-like phenotype. (A) The number of cells crossing the Transwell chamber membrane in each group was observed by inverted microscope after 72 h, and 5 fields were randomly selected to calculate the mean value of cells crossing the membrane; the right column diagram displays the statistical analysis of the number of invaded cells in each group, **P<0.01. (B) Bel7402 and HepG2 hepatoma cells were co-cultured with M0-NC or M0-AFP, and the effect of macrophages on apoptosis of HCC cells was detected by flow cytometry; the right column diagram displays the statistical analysis of the apoptosis rate of liver cancer cells, **P<0.01. The images are from the at least three independent experiments.