Fig. 14.

I-SHG imaging in muscle sarcomere adapted from Rivard et al. (2013a). a) F-SHG image in the absence of a reference SHG beam. b), c), d) raw I-SHG images acquired with a reference phase of 105°, 285°, and 465° respectively. Panels e) and f) images resulting from the subtractions of (c)—(b) and (d)—(c). Panel g) depicts the relative SHG phase in the muscle and (h) the histogram of the relative SHG phase for all pixels in (g)