Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 11;30(10):26308–26326. doi: 10.1007/s11356-022-23876-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — (I) Histopathology of Masson’s trichrome-stained sections of the testes. (A) Control group showing normal thin layer of tunica albuginea which is greenish in color (arrowhead). (B) Control group showing minimal amount of collagenous fibers in the interstitial tissue between the seminiferous tubules. (C) AgNP rats group injected with 1 mg for 30 days showing marked thickening of tunica albuginea (arrowheads). (D) AgNP rats group injected with 1 mg AgNPs for 30 days showing thickening of the basement membrane of the degenerated seminiferous tubules (arrow) with the presence of multiple spermatid giant cells (curved arrows). (E) AgNP rats group injected with 1 mg AgNPs for 30 days showing thickening of tubular basement membrane of completely degenerated and atrophied seminiferous tubules (arrow) with excessive amount of collagenous fibers in interstitial tissue (curved arrows). (Masson’s trichrome staining × 200), bar = 50µ. (II) Immunohistochemical expression of Bcl-2 and Caspase-3 in rats’ testicular tissues after exposure to 1 mg AgNPs for 30 days. (A) Control testes, showing strong positive cytoplasmic expression of Bcl-2 (arrows). (B) AgNP-treated group, showing negative expression of Bcl-2 (arrows) except for endothelium of small blood vessels in the interstitium. (C) Control testes, showing negative cytoplasmic immunolabeling of Caspase-3 (arrowheads) except for some Leydig cells (arrow). (D) AgNP-treated group, testes showing strong cytoplasmic labeling of Caspase-3 (arrowhead). Immunohistochemistry (IHC × 200, bar = 50µ)

Fig. 5 — (I) Histopathology of Masson’s trichrome-stained sections of the testes. (A) Control group showing normal thin layer of tunica albuginea which is greenish in color (arrowhead). (B) Control group showing minimal amount of collagenous fibers in the interstitial tissue between the seminiferous tubules. (C) AgNP rats group injected with 1 mg for 30 days showing marked thickening of tunica albuginea (arrowheads). (D) AgNP rats group injected with 1 mg AgNPs for 30 days showing thickening of the basement membrane of the degenerated seminiferous tubules (arrow) with the presence of multiple spermatid giant cells (curved arrows). (E) AgNP rats group injected with 1 mg AgNPs for 30 days showing thickening of tubular basement membrane of completely degenerated and atrophied seminiferous tubules (arrow) with excessive amount of collagenous fibers in interstitial tissue (curved arrows). (Masson’s trichrome staining × 200), bar = 50µ. (II) Immunohistochemical expression of Bcl-2 and Caspase-3 in rats’ testicular tissues after exposure to 1 mg AgNPs for 30 days. (A) Control testes, showing strong positive cytoplasmic expression of Bcl-2 (arrows). (B) AgNP-treated group, showing negative expression of Bcl-2 (arrows) except for endothelium of small blood vessels in the interstitium. (C) Control testes, showing negative cytoplasmic immunolabeling of Caspase-3 (arrowheads) except for some Leydig cells (arrow). (D) AgNP-treated group, testes showing strong cytoplasmic labeling of Caspase-3 (arrowhead). Immunohistochemistry (IHC × 200, bar = 50µ)