(A-D) Metabolic chambers were utilized to assess multiple components of energy balance, including energy expenditure (heat) (A-B), spontaneous activity (C), and respiratory exchange ratio (RER) (D); n=3–6 per group, *p<0.05, Dunnet’s test vs. WD Ctrl AA conducted separately for the light and dark cycles post 2-way RM ANOVA. (E) Glucose tolerance test (n=9/group, *p<0.05 vs. Ctrl AA, Dunnett’s test post ANOVA). (F) Insulin tolerance test (n=8–9/group, *p<0.05 vs. Ctrl AA, Dunnett’s test post ANOVA). Data represented as mean ± SEM.