(A-D) Metabolic chambers were utilized to assess multiple components of energy balance, including energy expenditure (heat) (A-B), spontaneous activity (C), and respiratory exchange ratio (RER) (D); n=8/group, *p<0.05, Dunnet’s test vs. WD Ctrl AA conducted separately for the light and dark cycles post 2-way RM ANOVA. (E-F) Fgf21 expression in the liver (E) and Ucp1 expression in the iWAT (F) of mice fed the indicated diets for 9 weeks (n = 6–9 per group, *p<0.05, Dunnett’s test vs. WD Ctrl AA post ANOVA). Data represented as mean ± SEM.