Table 2.

Diet composition of diets used to investigate the effect of reduced levels of essential amino acids in the context of a WD.

Amino Acid Defined Diets	Ctrl AA	WD Ctrl AA	WD Low AA	WD Low Phe	WD Low His
Teklad Diet name	21% Protein	AA Adj Calories, 21% Milkfat, 0.2% Chol	AA Adj Calories, 7% Protein, 21% Milkfat	Phe and Tyr Adj Western (Milkfat, Chol, Red)	His 2/3 Adj Western (Milkfat, Chol, Green)
Teklad Diet number	TD.140711	TD.160186	TD.160187	TD.171009	TD.171010
Color	Red	Aqua	Orange	Red	Green
Formula	g/kg	g/kg	g/kg	g/kg	g/kg
L-Alanine	9.38	9.38	3.05	9.966	9.8739
L-Arginine	6.3	6.3	2.05	6.3	6.3
L-Asparagine	20.58	20.58	6.7	21.1046	20.9462
L-Aspartic Acid	20.58	20.58	6.7	21.4557	21.318
L-Cysteine	7.2	7.2	2.34	7.2	7.2
L-Glutamic Acid	28.97	28.97	9.43	29.9377	29.79
L-Glutamine	33.77	33.77	11	34.2549	34.18
Glycine	2.96	2.96	0.96	3.4537	3.38
L-Histidine HCl, monohydrate	4.6	4.6	1.5	4.6	1.5
L-Isoleucine	7.8	7.8	2.54	7.8	7.8
L-Leucine	25.4	25.4	8.27	25.4	25.4
L-Lysine HCl	20.38	20.38	6.64	20.38	20.38
L-Methionine	6.7	6.7	2.18	6.7	6.7
L-Phenylalanine	6.6	6.6	2.15	2.15	6.6
L-Proline	7.41	7.41	2.41	8.167	8.05
L-Serine	7.41	7.41	2.41	8.1012	7.99
L-Threonine	9.7	9.7	3.16	9.7	9.7
L-Tryptophan	3.4	3.4	1.1	3.4	3.4
L-Tyrosine	6.9	6.9	2.25	2.25	6.9
L-Valine	8.4	8.4	2.735	8.4	8.4
Sucrose	291.248	341.46	341.46	341.46	341.46
Corn Starch	150.0	49.63	132.0625	51.5346	48.9509
Maltodextrin	150.0	49.63	132.0625	51.5346	48.9509
Anhydrous Milkfat	0.0	210	210	210	210
Cholesterol	0.0	1.5	1.5	1.5	1.5
Corn Oil	52.0	0.0	0.0	0.0	0.0
Olive Oil	29.0	0.0	0.0	0.0	0.0
Cellulose	30.0	50	50	50	50
Mineral Mix, AIN-93M-MX (94049)	35.0	35	35	35	35
Calcium Phosphate Ca(H2PO4)2 H2O	8.2	8.2	8.2	8.2	8.2
Vitamin Mix, Teklad (40060)	10.0	10	10	10	10
TBHQ, antioxidant	0.012	0.04	0.04	0.04	0.04
Food Coloring	0.1	0.1	0.1	0.1	0.1
% kcal from
Protein (based on N x 6.25)	22	20.7	6.8	18.9	19
Carbohydrates	59.4	38.5	52	39.5	39.2
Fat	18.6	40.9	41.2	41.6	41.8
Kcal/g	3.9	4.6	4.6	4.5	4.5