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. 2023 Feb 23;10:1139397. doi: 10.3389/fmed.2023.1139397

FIGURE 2.

FIGURE 2

Stimulation of collagen production by MC-EXO uptake in HLFs. (A) Secreted collagen was measured in the supernatants of HLF, HLF + MC-EXO (40 μg total protein), HLF + TGF-β (10 ng/ml), and HLF + bronchial epithelial cell exosomes (BEPI)-EXO (40 μg total protein) after 72 h. All assays performed in triplicate and normalized to total protein (μg). **P < 0.01 versus HLF, n = 5 samples/group. (B) Secreted collagen measured in the supernatants of HLF and HLF + MC-EXO (40 μg total protein) at the 48 h time point and then at the 72 h time point where the MC-EXO have been removed from the supernatant after 48 h. All assays performed in triplicate and normalized to total protein and normalized to total protein (μg). ***P < 0.001 versus HLF, n = 3 samples/group. (C) Secreted collagen measured in the supernatants of HLF and HLF with varying doses of MC-EXO. All assays performed in triplicate and normalized to total volume (700 μl). *P < 0.05, **P < 0.01. n = 3 samples/group.