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. 2023 Feb 23;14:1007624. doi: 10.3389/fimmu.2023.1007624

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Copyright © 2023 Zhou, Sun, Tang, Yuan, Wang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro validation of the targets using MTT assay and qPCR. (A) MTT assay to measure cell viability in PC12 cells after treatment with AU at 0 to 160 μM concentrations (In comparison with the control group, *P < 0.05, and ** P < 0.01). (B) MTT assay to measure PC12 cell proliferation after treatment with AU at 0 to 5 mM concentrations (In comparison with the control group, *P < 0.05, and ** P < 0.01). (C) Quantitative analysis of BTN3A2, AURKA, PLA2G7, ERAP2, CXCL10, MARCO, MAP-2, and βIII-tubulin gene expression in HFLS cells by real-time PCR (In comparison between two groups, *P < 0.05, and ** P < 0.01). (D) Quantitative analysis of BTN3A2, AURKA, PLA2G7, CXCL10, MARCO, MAP-2, and βIII-tubulin gene expression in PC12 cells by real-time PCR (In comparison between two groups, *P < 0.05, and ** P < 0.01).