Skip to main content
. 2023 Feb 23;14:1137332. doi: 10.3389/fimmu.2023.1137332

Figure 3.

Figure 3

Inhibition of HDAC8 blocks the activation of EGFR/ERK1/2/STAT3/HIF-1α signaling pathway in the peritoneum exposed to high glucose dialysate (A) Immunofluorescent co-staining of EGFR and HDAC8 (white arrows) in the peritoneum from mice with or without high glucose PDF injection. (B) Western blot analysis showed the protein levels of p-EGFR, EGFR, p-ERK1/2, ERK1/2, p-STAT3, STAT3, HIF-1α and GAPDH. Expression levels of (C) p-EGFR, (D) EGFR, (E) p-ERK1/2, (F) ERK1/2, (G) p-STAT3, (H) STAT3, (I) HIF-1α were quantified by densitometry and normalized with GAPDH, EGFR, ERK1/2 and STAT3 respectively. Data were expressed as means ± SEM. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. N.S., statistically not significant, with the comparisons labeled. All scale bars = 50 μm.