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. 2023 Feb 23;14:1137332. doi: 10.3389/fimmu.2023.1137332

Figure 8.

Figure 8

Blockade of HDAC8 with siRNA prevents M2 macrophage polarization via suppressing STAT6 and PI3K/Akt signaling pathways (A) Immunofluorescent staining of CD163 in Raw264.7 cells with different treatments (B–D) Serum-starved RAW264.7 were pretreated with siRNA and then exposed to IL-4 (10 ng/ml) for 24 h. Cell lysates were subjected to immunoblot analysis with specific antibodies against HDAC8, acetyl-cortactin, cortactin, CD163, Arg-1, p-STAT6, STAT6, p-PI3K, PI3K p-Akt, Akt and GAPDH. Expression levels of (E) HDAC8, (F) acetyl-cortactin, (G) cortactin, (H) CD163, (I) Arg-1, (J) p-STAT6, (K) p-PI3K, (L) p-Akt were quantified by densitometry and normalized with GAPDH, cortactin, STAT6, PI3K and Akt respectively. (M) Immunofluorescent staining of p-STAT6 in Raw264.7 cells with different treatments. Data were expressed as means ± SEM. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. N.S., statistically not significant, with the comparisons labeled. All scale bars = 50 μm.