Figure 6.

PKCε inhibitor regulates small-RhoGTPase activity. (A) High-density cortical neurons were treated with PKCε inhibitor for 24 h and OGD for 2 h. Cells were lysed for western blotting. Quantification of (B) PKCε; (C) total Rac1; (D) for total RhoA, and (E) for total CDC42. Activated form of (F) Rac1, (G) RhoA, and (H) CDC42 were measured using G-LISA kits. ^**P<0.01. n=3 for western blotting and n=5 for G-LISA. PKC, protein kinase C; Rac1, Rac Family Small GTPase 1; RhoA, Ras homolog gene family, member A; CDC42, cell division cycle 42.