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. 2023 Feb 23;11:1123024. doi: 10.3389/fpubh.2023.1123024

Table 2.

Rapid diagnostic test for Ebola virus.

Test Test type Virus detected Target Biological matrix Reference assay Sensitivity Specificity References
ReEBOV RDT (Corgenix, USA)** Immuno-chromatographic assay ZEBOV, SUDV, BDBV VP40 Whole EDTA blood, serum, plasma RealStar Filovirus Screen RT-PCR kit (Altona Diagnostics, Germany) Plasma: 93.2%* Whole EDTA blood: 98%. Plasma: 80.3%. Calculated using rRT-PCR as benchmark (55)
SD Q Line Ebola Zaire Ag test (SD Biosensor, Korea)** Immuno-precipitation lateral flow assay ZEBOV GP, NP, VP40 Plasma, serum and whole blood RealStar Filovirus Screen RT-PCR kit (Altona Diagnostics, Germany) Plasma: 84.50%* Whole EDTA blood: 100%. Plasma: 99.0%. Calculated using rRT-PCR as benchmark (55)
OraQuick Ebola Rapid Antigen Test (OraSure Technologies, USA)** Immuno-chromatographic lateral flow assay BDBV, ZEBOV, SUDV: VP40 Oral fluid and whole blood RT-PCR GeneXpert Ebola Test (Cepheid, USA) Whole blood: 57.4%* Whole blood: 98.3%. Calculated using rRT-PCR as benchmark (56)
Dual Path Platform (DPP) Ebola antigen system (Chembio, USA)** Immuno-chromatographic lateral flow assay ZEBOV VP40 Venous whole EDTA blood, venous plasma, serum and capillary fingerstick RT-PCR by Trombley et al. (43) Serum: 77.10%* Serum: 91.7% cross reactivity with malaria and other relevant HF pathogens (57)
QuickNavi-Ebola (Denka, Seiken, Tokyo, Japan)** Immuno-chromatographic assay ZEBOV, BDBV, TAFV: NP Whole blood and serum RT-PCR GeneXpert Ebola Test (Cepheid, USA) Whole blood: 85%* Whole blood: 99.8%. Calculated using r Gene Expert RT-PCR as benchmark (58)
E-ZYSCREEN RDT (CEA, Saclay, France) Lateral flow immunoassay ZEBOV GP Whole blood and serum RealStar Filovirus Screen RT-PCR kit (Altona Diagnostics, Germany) and RT-PCR by Weidmann et al. (46) Whole blood: 65.3%*. Serum: 74.5%* Whole blood: 98.8%. Serum: 100%. No cross reactivity with major hemorrhagic fever virus (59)
DEDIATEST Ebola (Senova, Germany) Lateral flow immunoassay ZEBOV VP40 Serum, throat swab, whole EDTA blood, plasma RealStar Filovirus Screen RT-PCR kit (Altona Diagnostics, Germany) Plasma: 79.5%* Whole EDTA blood: 100%. Plasma: 84.3%. Calculated using Altona rRT-PCR as benchmark (55)
One step Ebola test (Intec, China). Lateral flow immunoassay ZEBOV ND whole EDTA blood, plasma RealStar Filovirus Screen RT-PCR kit (Altona Diagnostics, Germany) Plasma: 98.4%* Whole EDTA blood: 95%. Plasma: 80.2%. Calculated using Altona rRT-PCR as benchmark (55)
Ebola Ag K-SeT (Coris BioConcept, Gembloux, Belgium) Immuno-chromatographic lateral flow assay ZEBOV VP40 Whole blood, plasma and serum RealStar Filovirus Screen RT-PCR kit (Altona Diagnostics, Germany) Plasma: 88.6%* Plasma: 98.1%. The specificity of the assay was validated with malaria, HIV, hepatitis viruses, Zika Virus, DENV3, and EBV (60)
NMRC EBOV LFI (Naval Medical Research Center for EBOV, Bethesda, USA) Lateral flow immunoassay SUDV, TAFV, RESTV GP Plasma, serum, oral swab RT-PCR by Trombley et al. (43) Plasma: 87.8%*. Oral swab: 88.9%* Plasma: 97.5%. Oral swab: 96.1%. Calculated using rRT-PCR as benchmark (61)

*The sensitivity of the tests was calculated using reference assay as benchmark, references (5561). **Current commercially available tests.

BDBV, Bundibugyo virus; DENV3, Dengue serotype 3; EBV, Epstein-Barr virus; GP, glycoprotein; HIV, Human Immunodeficiency Virus; NP, nucleoprotein; RESTV, Reston Ebola virus; SUDV, Sudan virus; TAFV, Tai Forest Ebola virus; VP40, viral protein 40; ZEBOV, Ebola virus Zaire; HF, Hemorrhagic Fever.