FIGURE 4.

Proliferative rate of neural progenitors in the ventricular zone (VZ). (A) [³H]-thymidine-labeled ([³H]-thy⁺) mitotic cells in contact with the lateral ventricle (LV) lumen were quantified 12 h following [³H]-thymidine administration. (B) Quantification of the percentage of [³H]-thy⁺ cells in mitosis relative to all [³H]-thy⁺ cells in the VZ 1.5, 6, 12, 24, and 72 h after [³H]-thymidine administration. (C) Doublet of non-mitotic [³H]-thy⁺ cells (asterisks) in the 1.5 h survival group. (D) Slow-dividing [³H]-thy⁺ type B cell (asterisk), corresponding to a uniciliated radial glia in contact with the LV lumen, 1 year after [³H]-thymidine administration. (E) BrdU/PCNA immunofluorescence detection in the sulcus medialis of an animal injected with BrdU with a survival time of 7 days. Most cells are double-positive, although some BrdU^–/PCNA⁺ and some BrdU⁺/PCNA^– cells were also detected. Scale bars: (A–C) 2 μm; (E) 25 μm.