FIG. 7.

IBP39 recognizes a single functional Inr motif. Mobility shift assays were performed with 10 ng of recombinant IBP39 and wild-type and mutant ferredoxin Inr probes. The sequences of the probes are listed below. (A) Recombinant IBP39 binds the ferredoxin single Inr element. Lanes 1 to 3, wild-type ferredoxin Inr; lanes 4 to 6, +1 A-to-G mutation; lanes 7 to 9, +1 A-to-T mutation; lanes 10 to 12, −5 A-to-G mutation. Lanes 1, 4, 7, and 10 are DNA probe-only controls. (B) Mutation of non-Inr sequences just upstream of the ferredoxin Inr does not affect IBP39 binding. Lanes 1 to 3, −3 and −4 TC-to-CT mutation; lanes 4 to 6, −3 and −4 TC-to-AG mutation; lanes 7 to 9, wild-type ferredoxin Inr. Lanes 1, 4, and 7 are DNA probe-only controls.