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. 2023 Feb 23;14:1125594. doi: 10.3389/fimmu.2023.1125594

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Copyright © 2023 Wolfschmitt, Hogg, Vogt, Zink, Wachter, Hezel, Zhang, Hoffmann, Gröger, Hartmann, Gässler, Datzmann, Merz, Hellmann, Kranz, Calzia, Radermacher and Messerer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of Na₂S₂O₃ on porcine PBMCs. Metabolic fluxes calculated from ¹³C labeling patterns on metabolites. PBMCs are displayed as circles and granulocytes as triangles. Animals were treated either with vehicle (n = 8, bright symbols) or Na₂S₂O₃ (n = 9, dark symbols). (A) F4 trends in PBMCs in vehicle vs Na₂S₂O₃-treated animals and correlations between the difference in F4 and the difference in routine respiration over time. (B) Acetyl-CoA loss of PBMCs and granulocytes. Correlation between the difference in F4 and the difference in Acetyl-CoA loss over time. Significant P values are indicated in the graphs; Mann-Whitney U test for intergroup differences, Kruskal-Wallis rank sum test for time-related effects. Data are presented as median with IQR. Correlations are presented with 95% confidence intervals.