TABLE 5.
Effects of deletion of SR09 on Hap1 activitya
| Strains | β-Galactoside activity (Miller units) when grown in the presence of the indicated concentration of dpIX
|
||||||
|---|---|---|---|---|---|---|---|
| 0 μg/ml | 0.031 μg/ml | 0.062 μg/ml | 0.125 μg/ml | 0.25 μg/ml | 0.5 μg/ml | 1.0 μg/ml | |
| Wild type | 0.6 ± 0.1 | 14 ± 2 | 38 ± 5 | 339 ± 100 | 625 ± 133 | 876 ± 102 | 1,266 ± 94 |
| Δsro9 | 8.0 ± 0.4 | 35 ± 3 | 119 ± 8 | 437 ± 64 | 617 ± 130 | 755 ± 104 | 984 ± 40 |
Yeast Δsro9 Δhem1 Hap1-expressing cells bearing the UAS1/CYC1-lacZ reporter were grown in the presence of the indicated concentrations of heme analogue dpIX. β-Galactosidase activities were detected, and data shown are averages (± standard deviations) of at least three independent transformants.