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. 2023 Feb 15;25(4):134. doi: 10.3892/ol.2023.13720

Figure 4.

Figure 4.

Association between FOXA1 and LPCAT1. (A) UALCAN database analysis based on The Cancer Genome Atlas data indicates that FOXA1 is highly expressed in breast cancer tissues. ***P<0.001 vs. normal. Expression levels of FOXA1 in MCF-10A and MDA-MB-231 cells were assessed using (B) RT-qPCR and (C) western blotting. ***P<0.001 vs. MCF-10A. Expression of FOXA1 in the MDA-MB-231 cells transfected with oe-FOXA1 was confirmed using (D) RT-qPCR and (E) western blotting. Expression of LPCAT1 in the transfected MDA-MB-231 cells was confirmed using (F) RT-qPCR and (G) western blotting. ***P<0.001 vs. oe-NC. (H) Binding sites for transcription factors FOXA1 and LPCAT1 promoters predicted using the HumanTFDB website. (I) LPCAT1 promoter activity was determined with a luciferase reporter assay. ***P<0.001 vs. LPCAT1 + oe-NC (WT). (J) Binding between FOXA1 and LPCAT1 was evaluated with a chromatin immunoprecipitation assay. ***P<0.001 vs. IgG. FOXA1, forkhead box A1; LPCAT1, lysophosphatidylcholine acyltransferase 1; RT-qPCR, reverse transcription-quantitative PCR; HumanTFDB, Human Transcription factor Database; oe, overexpression; NC, negative control; BRCA, breast invasive carcinoma; WT, wild type; MUT, mutant.