Table 1.
Summary of extraction methods. The kit cost is the cost of the kit per reaction (rxn), whereas the total cost is the cost of one reaction, including kit cost, laboratory reagents and consumables. The kit cost for MPIH includes the cost of a few additional reagents due to increased amounts of these reagents being used compared to the manufacturer’s protocol
|
Code |
Full kit name and company |
Method type |
Amount of culture (ml) |
Kit cost (CAD, per rxn) |
Total cost (CAD, per rxn) |
Total time (12 rxn) |
Hands-on time (12 rxn) |
|---|---|---|---|---|---|---|---|
|
MP |
MasterPure Complete DNA and RNA Purification kit (Lucigen) |
Salt precipitation |
0.5 |
$4.19 |
$6.56 |
1 h 55 m |
1 h 25 m |
|
OMEGA |
E.Z.N.A. Bacterial DNA Kit (OMEGA) |
Silica column |
1.0 |
$7.33 |
$10.32 |
2 h 20 m |
1 h 20 m |
|
QIA |
DNeasy Blood and Tissue Kit (Qiagen) |
Silica column |
0.5 |
$3.54 |
$5.99 |
2h |
1h |
|
GE |
Illustra Bacteria genomicPrep Mini Spin Kit (GE) |
Silica column |
1.0 |
$5.69 |
$7.86 |
1 h 10 m |
1 h 10 m |
|
EZ1 |
EZ1 DNA Tissue Kit (Qiagen) |
Magnetic particles |
1.0 |
$11.25 |
$12.32 |
3 h 35 m |
35 m |
|
MPIH |
Modified, in house MasterPure Kit |
Salt precipitation |
0.5 |
$5.34 |
$8.12 |
1 h 55 m |
1 h 40 m |