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IR-61 binds with Keap1 Protein. Recombinant human Keap1 protein was incubated with IR-61 in 1 M HEPES buffer (pH 7.4) at 37 °C for 30 min, and then the mixture was subjected to western blot. NIR fluorescence band was detected (left panel) and then incubated with Keap1 antibody to scan the protein band (right panel).
