Reagent/resource	Reference or source	Identifier or catalog
Bacterial strains
Mycobacterium bovis BCG Pasteur	American Type Culture Collection	ATCC 35748
M. bovis BCG gltBD transposon mutant	VIB – Vlaams Instituut voor Biotechnologie	(BCG_3922c TnInsertion‐8654)
Reagents
Middlebrook 7H11	Merck Life Science	M0428‐500G
Middlebrook 7H9	Merck Life Science	M0178‐500G
OADC	Becton Dickenson	212351 (4312351)
Glycerol	Merck Life Science	G7893‐1 L
Tyloxapol	Merck Life Science	T8761‐50G
Brain heart infusion agar	Merck Life Science	70138‐500G
Rosins minimal media	Beste et al (2011)	N/A
[13C3] glycerol, 99% purity	CK Isotopes	CLM‐1510‐5
[15N1] NH4Cl, 98% atom purity	Merck Life Science	299251
2 liter bioreactor	Electrolab	Fermac 310/60
Peristaltic pump	Rainin Rabbit Plus	N/A
Gas analyzer	Electrolab	Fermac 368
Glycerol assay kit	Merck Life Science	MAK117
Ammonia assay kit	Merck Life Science	MAK310
BCA assay kit	Merck Life Science	71285‐M
Ziehl neelsen stain	Merck Life Science	1.09215
Mass spectrometry equipment
tert‐butyldimethyl silyl chloride (TBDMSCl)	Merck Life Science	00942‐10ML
N‐Methyl‐N‐(trimethylsilyl)trifluoroacetamide, MSTFA	Merck Life Science	M‐132
GC–MS 7890‐5795	Agilent	Borah et al (2019)
VF‐5 ms 30 m × 0.25 mm × 0.25 μm + 10 m EZ‐Guard	Agilent	CP9013
Dionex UltiMate system (HPLC)	Thermo Fisher Scientific	3000 RSLC
C18 and ZIC‐pHILIC column (150 mm × 4.6 mm, 5 μm column)	Merck Sequant	150461
Thermo Orbitrap Q Exactive Plus	Thermo Fisher Scientific	N/A
Software
Chemstation	Agilent	N/A
GraphPad Prism 8.0	GraphPad software	N/A
Omix v.2.0.7	Omix Visualization GmbH & Co.KG, Lennestadt/Germany	N/A
13CFLUX2 v2.2	Weitzel et al (2013)	N/A
HOPS v2.0.0	Jadebeck et al (2021)	N/A