Table 1.
Detail of polymerase chain reaction primers utilized in this study and their polymerase chain reaction reaction assay
| Gene name | Reference | Sequence (5′-3′) | Primer length (nt) | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
| CircRNA-ZNF638 (Divergent primers) | Hui et al [2] | F:TTTGCAATGCCCAGGTTCAA, | 20 | 250 | 55 |
| R:CTGACATCCCCATTCGGTCT | 20 | ||||
| CK6 | XM_018047719.1 in GenBank | F:AGTTTGCCTCCTTCATCG, | 18 | 111 | 53 |
| R:GGTTCTGCTTCACGGTCTT | 19 | ||||
| Ki67 | XM_005698601.3 in GenBank | F:AGGAAGTAGCCAGACTGAGGG, | 21 | 143 | 56 |
| R:GCATCGTGGTTTGCTGTGAA | 20 | ||||
| Sox9 | XM_018063905.1 in GenBank | F:GGTGCTCAAGGGCTACGACTGG | 22 | 162 | 60 |
| R:GCGTTGTGCAGGTGCGGGTA | 20 | ||||
| CD34 | XM_018060788.1 in GenBank | F:GAAGATGTCAGCAGCCACCAG | 21 | 112 | 56 |
| R:GGCGGTTCATCAGGAAATAGCAC | 23 | ||||
| CD200 | XM_005674916.3 in GenBank | F:TTGGAAGATGAGGCGTGTTA | 20 | 156 | 54 |
| R:AGCATTGGCAGAGCAAGTGA | 20 | ||||
| GAPDH | XM_005680968.3 | F:TGAACCACGAGAAGTATAACAACA | 24 | 125 | 53 |
| R:GGTCATAAGTCCCTCCACGAT | 21 | ||||
| CircRNA-ZNF638 (Me-RIP-qPCR) | This study | F: TCATTGTCTGATTAACTGTCTGGCT | 25 | 167 | 53 |
| R: GTTTCGACAGATGGACTTCCC | 21 | ||||
| miR-361-5p | MIMAT0036171 in miRNAsong | F: GCTTATCAGAATCTCCAGGGGTAC | 24 | NA | 60 |
| U6 | Han et al [20] | F: CGCTTCGGCAGCACATATAC | 20 | NA | 55 |
| R:AAATATGGAACGCTTCACGA | 20 | ||||
| Wnt5a | XM_018038229.1 | F: ACATCACTTGGCGAATGGACG | 21 | 118 | 57 |
| R: CTGGGAGCAGTTCTCGGGACA | 21 |
CircRNA-ZNF638, circRNA-zinc finger protein 638; CK6, cytokeratin 6; Ki67, marker of proliferation Ki-67; Sox9, SRY-box transcription factor 9; CD34, CD34 molecule; CD200, CD200 molecule; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; U6, U6 small nuclear RNA; Wnt5a, Wnt family member 5A; NA, not available.