Figure 1. Effect of IAA on the segregation of the ASH1 mRNA to daughter cells .

A) Schematic illustration of the AID system. Briefly, a protein of interest is tagged with the AID motif in a cell that is simultaneously expressing the E3 ubiquitin ligase subunit TIR1. Auxin addition then causes TIR1 to interact with the AID motif, targeting the AID-tagged protein for ubiquitination, as this will bring ubiquitin (Ub)-activating E2 enzymes into proximity. Ubiquitination of the protein of interest will target it for proteasomal degradation. B) Top: Representative images of yeast cells displaying ASH1 mRNA foci in the mother, in the daughter, and in the neck. The ASH1 mRNA particles are observed as foci, indicated by yellow arrowheads. Bottom: Quantitative analysis of the distribution of mRNA foci located in the mother, in the neck, or in the daughter in the absence of IAA or after the indicated times after its addition. At least 50 foci, and never less than 400 cells, were counted per condition, timepoint, and experiment. The bar height represents the mean value out of 3 independent experiments and error bars indicate the standard error of that mean. The p -value (ns = non-significant) was derived by unpaired one-way ANOVA between the concerned means.