Reagent or resource	Source	Identifier
Experimental models: Cell lines
AICS-0014 cl. 6, nucleoli (DFC)	https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref = AICS-0014&Product = iPSC	CVCL_JM17
Chemicals, peptides, and recombinant proteins
mTeSR™1 Medium Without Phenol Red	STEMCELL Technologies	Cat#05876
mTeSR™1 Medium	STEMCELL Technologies	Cat#85850
DPBS (1X)	Thermo Fischer Scientific	Cat#14190144
StemPro™ Accutase™ Cell Dissociation Reagent	Thermo Fischer Scientific	Cat#A1110501
CellMaskTM Deep Red Plasma Membrane Stain	Thermo Fisher Scientific	Cat#C10046; Lot#1813792 (5X final concentration); Lot#1853335 and #1900978 (3X final concentration)
NucBlueTM Live ReadyProbesTM Reagent	Thermo Fisher Scientific	Cat#R37605
Alexa Fluor® 647 Mouse anti-Human Nanog, Clone N31-355	BD Biosciences	AB_10611718; Cat#561300