Category	Reagent or resource	Source	Identifier
Stand	Observer.Z1 microscope stand	Zeiss
Objectives	10X/0.45 NA Plan-Apochromat objective, FWD = 2.1mm	Zeiss	Cat#420640-9900-000
	100X/1.25 W C-Apochromat Korr UV Vis IR objective, FWD = 0.25mm	Zeiss	Cat#421797-9970-000
Advanced modality	Spinning disk head CSU-X with Primary dichroic RQFT 405/488/568/647 BP filter	Yokogawa	M1N-E/FBO/C101; 24V DC; P8X006; 95P900140
Emission filters	NucBlue Live dye: BP filter 450/50	Chroma	Cat#ET450/50m
	mEGFP tag structure or bright field: BP filter 526/50	SEMROCK	Cat#FF03-525/50
	CMDR dye: BP filter 690/50	Chroma	Cat#690/50m
	mTagRFP-T tag structure: BP filter 600/50	Chroma	Cat#ET600/50m
	Bright field: BP filter 706/95	Chroma	Cat#ET706/95m
Fluorescence Excitation light source	Laser: LASOS 405 50mw	Zeiss	Part#400600-9011-000
	Laser: LASOS 488 100mw	Zeiss	Part#400600-9061-000
	Laser: LASOS 561 75mw	Zeiss	Part#400600-9111-000
	Laser: LASOS 638 75mw	Zeiss	Part#400600-9121-000
Transmitted light source	Transmitted light red; LAMBDA TLED+ with 740 nm wavelength LED	Sutter Instruments
	Transmitted light white; Attachment lamp VIS-LED (400–700 nm) with collector for laser system	Zeiss	Part#423053-9060
Detector	Orca Flash 4.0 V2+ cameras	Hamamatsu	Part#C1144-22CU
Stage components	Piezo z-drive: Prior NanoScan Z 100 mm piezo z-stage: NZ100ZM/a	Zeiss	Part#2802000 224
	PECON Incubator XLmulti S1; (heat and CO2 control: 37°C with 5% CO2)	Zeiss	Part#2802000 224
	Stage insert: H201 k frame slim profile model	Okolab	Part#H201 k