Figure 1.

22-mer, 25-mer, and 30-mer PMO structures

Shown for three therapeutic PMOs, Exon45 (22-mer PMO), Exon53 (25-mer PMO), and Exon51 (30-mer PMO), are the primary sequence (A) and the reference structures: initial extended conformations (B) and unfolded conformations (C). The PMOs represent therapeutic oligomers complementary to the different indicated exon transcripts in the human dystrophin gene. Each sequence is shown with its base composition, the total amount of nucleobases, i.e., adenine, A; cytosine, C; guanine, G; and thymine, T, and the relative amount of G bolded (percentage). The conformers are shown in licorice representation (sticks) and in twister representation (blue line) describing the backbone. The MPG linker is shown in orange (within the black circle). The A and T bases are shown in green color, whereas C and G bases are shown in red.