Table 1.

Solution conformations of therapeutic 22-mer PMO for Exon45, 25-mer PMO for Exon53, and 30-mer PMO for Exon51

22-mer PMO
	$\mathrm{w}$	$X$, nm	$R_g$, nm	$N_{BP}$	$N_{BS}$	SASA, Å2	$\left[\eta \right]$, cm3/g	$\Delta \mathrm{G}$, kcal/mol	$T\Delta S$, kcal/mol	$\Delta H$, kcal/mol
I	0.23	1.75	1.76	3	4	4,665	5.35	−58	−19	−75
II	0.22	0.81	1.31	3	8	4,368	3.88	−18	−28	−46
III	0.16	1.66	1.50	4	4	4,912	4.72	−23	−21	−44
25-mer PMO
	$\mathrm{w}$	$X$, nm	$R_g$, nm	$N_{BP}$	$N_{BS}$	SASA, Å2	$\left[\eta \right]$, cm3/g	$\Delta \mathrm{G}$, kcal/mol	$T\Delta S$, kcal/mol	$\Delta H$, kcal/mol
I	0.19	2.1	1.25	6	12	4,803	5.8	−71	−43	−114
II	0.17	2.5	1.22	5	6	4,602	3.9	−33	−46	−79
III	0.16	3.9	1.37	6	11	5,002	4.5	−55	−32	−87
30-mer PMO
	$\mathrm{w}$	$X$, nm	$R_g$, nm	$N_{BP}$	$N_{BS}$	SASA, Å2	$\left[\eta \right]$, cm3/g	$\Delta \mathrm{G}$, kcal/mol	$T\Delta S$, kcal/mol	$\Delta H$, kcal/mol
I	0.25	3.7	1.60	12	7	5,651	5.7	−53	−60	−113
II	0.13	1.7	1.59	5	12	5,794	5.7	−54	−58	−112
III	0.12	2.6	1.71	3	5	6,299	6.7	−51	−34	−85

Shown for each principal solution conformer I–III are the equilibrium population $w$, end-to-end distance $X$, radius of gyration $R_g$, number of base pairs $N_{BP}$, numbers of base stacks $N_{BS}$, SASA, intrinsic viscosity $\left[\eta \right]\text{,}$ and thermodynamic state functions (changes in free energy $\Delta G$, entropy $T\Delta S$, and enthalpy $\Delta H$) for folding of PMOs.