Genetic manipulation permits tunability of tissue-derived hydrogels. (A) Schematic of hydrogel preparation and example macroscopic image of hydrogel. (B) Optical density during gelation and (C) the time to half gelation of 4 mg/mL hydrogels (n = 5). (D–F) Representative SEM images of (D) WT, (E) 1:1, and (F) TSP-2 KO gel show similar structures. Scale bars = 5 μm. (G) Rheology reveals overall changes in maxima and suggests tunability of mechanical properties between WT, TSP-2 KO, and a 1:1 mixture (rheological traces are given as mean ± SEM). (H) Analysis of rheological data indicates significant changes between maximum storage moduli with genotype of matrix (n = 3). Results are given as mean + SEM, *p < 0.05, **p < 0.01, ***p < 0.005.