FIG. 4.

GGA domains are differentially required for α-factor maturation. (A) Complementation of gga1Δ gga2Δ α-factor secretion defect by GGA2 truncations. Strains described for Fig. 3 were spotted on a lawn of tester strain RC634 (MATa sst1-3). Relative growth inhibition of tester lawn is indicative of level of mature α-factor secretion. (B) Complementation of gga1Δ gga2Δ α-factor-processing defect by GGA2 truncations. Strains described for panel A were pulse-labeled, and processed and mature forms of α-factor were immunoprecipitated using anti-α-factor antibodies. Immunoprecipitated proteins were resolved by SDS-PAGE and analyzed, and forms of α-factor and molecular mass standards are indicated as in Fig. 2B.