TABLE 2.
Plasmids used in this study
| Plasmid | Descriptiona | Reference |
|---|---|---|
| pCM54B4 | CEN-URA plasmid encoding Gga1p-HA (pYX112 based) | This study |
| pCM89B2 | CEN-URA plasmid encoding Gga1p-HA containing CBS#1 and CBS#2 mutations (pYX112 based) | This study |
| pCM123B3 | CEN-URA plasmid encoding Gga1p-ΔGAE (aa 445–557)-HA (pYX112 based) | This study |
| pCM124B5 | CEN-URA plasmid encoding Gga1p-ΔGAE (aa 445–557)-HA containing CBS#1 and CBS#2 mutations (pYX112 based) | This study |
| pCM53-3 | CEN-URA plasmid encoding Gga2p-HA (pYX112 based) | This study |
| pCM90-3 | CEN-URA plasmid encoding Gga2p-HA containing CBS#1 mutation (pYX112 based) | This study |
| pCM62-2 | CEN-URA plasmid encoding Gga2p-ΔGAE (aa 472–585)-HA (pYX112 based) | This study |
| pCM126-4 | CEN-URA plasmid encoding Gga2p-ΔGAE (aa 472–585)-HA containing CBS#1 mutation (pYX112 based) | This study |
| pCM63-1 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 337–585)-HA (pYX112 based) | This study |
| pCM64-1 | CEN-URA plasmid encoding Gga2p-ΔVHS (aa 1–169)-HA (pYX112 based) | This study |
| pCM65-2 | CEN-URA plasmid encoding Gga2p-ΔVHS/GAT (aa 1–336)-HA (pYX112 based) | This study |
| pCM135-1 | CEN-URA plasmid encoding Gga2p-ΔVHS (aa 1–39)-HA (pYX112 based) | This study |
| pCM136-3 | CEN-URA plasmid encoding Gga2p-ΔVHS (aa 1–77)-HA (pYX112 based) | This study |
| pCM137-2 | CEN-URA plasmid encoding Gga2p-ΔVHS (aa 1–97)-HA (pYX112 based) | This study |
| pCM138-1 | CEN-URA plasmid encoding Gga2p-ΔVHS (aa 1–132)-HA (pYX112 based) | This study |
| pCM139-1 | CEN-URA plasmid encoding Gga2p-ΔVHS (aa 1–155)-HA (pYX112 based) | This study |
| pCM142B-3 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 442–585) (pYX112 based) | This study |
| pCM143B-9 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 409–585) (pYX112 based) | This study |
| pCM144B-4 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 370–585) (pYX112 based) | This study |
| pCM146-16 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 409–585) containing CBS#1 mutation (pYX112 based) | This study |
| pCM147-8 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 370–585) containing CBS#1 mutation (pYX112 based) | This study |
| pCM85-1 | CEN-URA plasmid encoding Gga1-N215A (pYX112 based) | This study |
| pCM86-5 | CEN-URA plasmid encoding Gga2-N219A (pYX112 based) | This study |
| pCM147-8 | CEN-URA plasmid encoding Gga2p-Δhinge/GAE (aa 370–585) containing CBS#1 mutation (pYX112 based) | This study |
| pCM146-1 | CEN-URA plasmid encoding Gga2p-hinge/GAE (aa 337–585) containing CBS#1 mutation (pYX112 based) | This study |
| pCM133-2 | CEN-URA plasmid encoding Kex2p-HA (pYX112 based) | This study |
| pCM102-2 | GST fusion vector pGEX-5X-1 encoding GST-Gga1p-VHS/GAT (aa 1–331) | This study |
| pCM24-1 | GST fusion vector pGEX-5X-1 encoding GST-Gga1p-hinge/GAE (aa 332–557) | This study |
| pCM3-1 | GST fusion vector pGEX-5X-1 encoding GST-Gga1p-hinge/GAE (aa 332–557) containing CBS#1 mutation | This study |
| pCM8-3 | GST fusion vector pGEX-5X-1 encoding GST-Gga1p-hinge/GAE (aa 332–557) containing CBS#2 mutation | This study |
| pCM9-2 | GST fusion vector pGEX-5X-1 encoding GST-Gga1p-hinge/GAE (aa 332–557) containing CBS#1 and CBS#2 mutations | This study |
| pCM103-3 | GST fusion vector pGEX-5X-1 encoding GST-Gga2p-VHS/GAT (aa 1–336) | This study |
| pCM43-5 | GST fusion vector pGEX-5X-1 encoding GST-Gga2p-hinge/GAE (aa 337–585) | This study |
| pCM98-1 | GST fusion vector pGEX-5X-1 encoding GST-Gga2p-hinge/GAE (aa 337–585) containing CBS#1 mutation | This study |
| pCM151B-1 | GST fusion vector pGEX-5X-1 encoding GST-Gga2p-GAE (aa 472–585) | This study |
| pCM152B-1 | GST fusion vector pGEX-5X-1 encoding GST-Gga2p-Δhinge/GAE (aa 357–585) | This study |
a
Plasmids are described relative to full-length or mutant proteins that they encode. Amino acid residues indicated in parentheses for plasmids encoding Gga1p and Gga2p truncations indicate the residues deleted, while those indicated in parentheses for GST fusion constructs indicate the residues of Gga1p or Gga2p fused to GST. .