TABLE 3.
Percentages of CPY missorting in cells expressing wild-type and mutant GGA1 and GGA2-encoded proteins
| Strain (plasmid) | % ± SEMa | No. of trials |
|---|---|---|
| Wild type (vector) | 0.5 ± 0.3 | 17 |
| gga1Δ gga2Δ (vector) | 100.0 | |
| gga1Δ gga2Δ (GGA2) | 0.7 ± 0.2 | 12 |
| gga1Δ gga2Δ (gga2-ΔGAE) | 4.5 ± 1.3 | 7 |
| gga1Δ gga2Δ (gga2-Δhinge/GAE) | 34.7 ± 2.0 | 10 |
| gga1Δ gga2Δ (gga2-ΔVHS) | 72.5 ± 1.9 | 9 |
| gga1Δ gga2Δ (gga2-ΔVHS/GAT) | 101.4 ± 1.9 | 5 |
| gga1Δ gga2Δ (gga1-N215A) | 13.5 ± 2.2 | 10 |
| gga1Δ gga2Δ (gga2-N219A) | 25.9 ± 3.6 | 10 |
| Wild type (gga2-ΔVHS/GAT) | 1.4 ± 0.7 | 4 |
| Wild type (gga2-ΔVHS/GAT + CBS#1) | 1.6 ± 0.7 | 4 |
| gga1Δ gga2Δ (GGA1) | 0.6 ± 0.5 | 8 |
| gga1Δ gga2Δ (gga1-CBS#1-CBS#2) | 0.9 ± 0.5 | 3 |
| gga1Δ gga2Δ (gga1-ΔGAE) | 4.3 ± 0.3 | 4 |
| gga1Δ gga2Δ (gga1-ΔGAE + CBS#1-CBS#2) | 13.6 ± 2.3 | 5 |
| gga1Δ gga2Δ (gga2-CBS#1) | 0.9 ± 0.3 | 4 |
| gga1Δ gga2Δ (gga2-ΔGAE + CBS#1) | 13.6 ± 3.3 | 4 |
| gga1Δ gga2Δ (gga2-Δh[442–471]/GAE) | 4.3 ± 0.3 | 3 |
| gga1Δ gga2Δ (gga2-Δh[409–471]/GAE) | 2.0 ± 0.3 | 3 |
| gga1Δ gga2Δ (gga2-Δh[370–471]/GAE) | 5.2 ± 1.2 | 3 |
| gga1Δ gga2Δ (gga2-Δh[409–471]/GAE + CBS#1) | 11.7 ± 0.6 | 9 |
| gga1Δ gga2Δ (gga2-Δh[370–471]/GAE + CBS#1) | 28.6 ± 1.9 | 9 |
a
See Materials and Methods for method of calculating percent CPY missorting and standard error of the mean (SEM).