TABLE 2.

Microfluidic applications for microbial and mammalian cells based on culture modes

Culture modes	Application	Cell types	Remarks
Batch	Screening for desired phenotypes	Escherichia coli	[138]
		Saccharomyces cerevisiae	[138]
		Pichia pastoris	[43]
		Yarrowia lipolytica	[42]
		Yeast	[32]
		Aspergillus niger	[33]
		Hybridoma cells	[44, 132]
	Antimicrobial susceptibility testing (AST)	Escherichia coli	[39, 40]
		Klebsiella pneumoniae	[40]
		Staphylococcus aureus	[40, 139]
		Enterococcus faecalis	[40]
	Cell migration	Human breast cancer cell lines, human neutrophils	[140]
	Cell‐cell interaction	Clonostachys rosea, Fusarium graminearum	[141]
	Determination of monod kinetic parameter	Saccharomyces cerevisiae	[24, 142]
Fed‐Batch	Multidrug screening	A549 cells	[143]
	Optimise feeding strategies	Corynebacterium glutamicum	[18]
Perfusion	Bioprocess development	Pichia pastoris	[27]
		Saccharomyces cerevisiae	[21]
		CHO cell line	[97, 144]
		C2C12, HFF cell lines and mouse embryonic stem cells	[145]
	Cell migration	Pseudomonas putida	[146]
		Human oral squamous cancer cell line, Human glioblastoma cell line	[147]
		Jurkat cell	[148]
	Cytotoxicity assay	HUVEC cells	[61]
Subculture	Adaptive evolution	Escherichia coli	[34, 126]
3D culture	Cytotoxicity assay	HeLa cells	[149]
		Colon cancer cells (HCT116)	[81]
	Cell‐cell interaction	Tumour‐endothelial cell	[118]