Table 1.
Original p values from the generalized linear model (GLM) of the synovial fluid (SF) and its extracellular vesicle-enriched pellet (EV) fatty acids (FAs) and the calculated Benjamini–Hochberg (B–H) critical values used to control the false discovery rate
| SF FA | p group | SF FA | p group × age | SF B–H critical value |
EV FA | p group | EV FA | p group × age | EV B–H critical value |
|---|---|---|---|---|---|---|---|---|---|
| n-3/n-6 PUFAs | 3 × 10–14* | n-3/n-6 PUFAs | 3 × 10–12* | 0.0005 | 18:0 | 0.002 | 22:0 | 0.002 | 0.0006 |
| 16:1n-7 | 7 × 10–11* | 16:1n-7 | 1 × 10–8* | 0.0011 | 22:0 | 0.003 | 18:0 | 0.004 | 0.0011 |
| 17:0i | 3 × 10–8* | 17:0i | 1.5 × 10–7* | 0.0016 | 16:0 | 0.020 | 16:1n-7 | 0.032 | 0.0017 |
| Prod/prec n-6 | 0.00008* | Prod/prec n-6 | 0.0005* | 0.0022 | ∆5-DI n-6 | 0.040 | 16:0 | 0.077 | 0.0022 |
| 18:2n-6 | 0.0006* | 16:0i | 0.0024* | 0.0027 | 22:1n-9 | 0.040 | 22:1n-9 | 0.079 | 0.0028 |
| 18:0i | 0.001* | 18:0i | 0.0031* | 0.0033 | 20:4n-6 | 0.088 | 18:2n-6 | 0.097 | 0.0033 |
| 16:0i | 0.001* | 18:2n-6 | 0.0037* | 0.0038 | 16:1n-9 | 0.091 | 18:3n-3 | 0.109 | 0.0039 |
| ∆5-DI n-6 | 0.003* | 24:1n-9 | 0.0039* | 0.0043 | 16:1n-7 | 0.093 | Prod/prec n-3 | 0.139 | 0.0044 |
| 14:0 | 0.003* | 22:0 | 0.0047* | 0.0049 | 17:0i | 0.108 | 15:0 | 0.162 | 0.0050 |
| n-6 PUFAs | 0.004* | ∆5-DI n-6 | 0.010 | 0.0054 | 15:0 | 0.109 | 16:1n-9 | 0.187 | 0.0056 |
| 22:0 | 0.004* | n-6 PUFAs | 0.012 | 0.0060 | 18:2n-6 | 0.121 | ∆5-DI n-6 | 0.202 | 0.0061 |
| DMA 18:0 | 0.005* | DMA 18:0 | 0.015 | 0.0065 | 20:0 | 0.130 | 20:0 | 0.205 | 0.0067 |
| 24:1n-9 | 0.013 | 20:3n-6 | 0.020 | 0.0071 | DMA 18:0 | 0.132 | DBI | 0.222 | 0.0072 |
| ∆6-DI n-6 | 0.019 | n-3 PUFAs | 0.029 | 0.0076 | 18:3n-3 | 0.133 | SFAs | 0.284 | 0.0078 |
| n-3 PUFAs | 0.023 | 14:0 | 0.029 | 0.0082 | DMAs | 0.172 | UFAs/SFAs | 0.312 | 0.0083 |
| 18:3n-6 | 0.032 | 18:3n-6 | 0.046 | 0.0087 | 24:0 | 0.198 | DMAs | 0.355 | 0.0089 |
| 20:3n-6 | 0.033 | Prod/prec n-3 | 0.054 | 0.0092 | Prod/prec n-3 | 0.199 | 17:0ai | 0.412 | 0.0094 |
| 17:0ai | 0.043 | 22:5n-3 | 0.054 | 0.0098 | DBI | 0.208 | 20:4n-6 | 0.437 | 0.0100 |
The p values that remained significant after the procedure were obtained by comparing the original p value to the corresponding critical value on the same row. Asterisk indicates comparisons for which the direction of the difference is confidently interpreted at the α/2 level. The SF FAs that originally had p values >0.05 in the GLM were excluded from the table. In the EV fraction, no significant differences remained after the procedure, but a similar number of FAs is shown
ai anteiso-methyl-branch, DBI double bond index, DI desaturation index, DMA dimethyl acetal, i iso-methyl-branch, Prod/prec product/precursor ratio, PUFA polyunsaturated fatty acid, SFA saturated fatty acid, UFA unsaturated fatty acid