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. 2022 Nov 24;130(2):202–216. doi: 10.1016/j.bja.2022.09.027

Fig 5.

Fig 5

Intrathecal (i.t.) administration of nerve injury-specific long non-coding RNA (NIS-lncRNA) antisense oligonucleotides (ASOs, 100 μg) blocked the increases of C–C chemokine ligand 2 (Ccl2) mRNA and CCL2 protein in dorsal root ganglion (DRG) under conditions of neuropathic pain caused by chronic constriction injury (CCI), spinal nerve ligation (SNL), paclitaxel (PTX) injection, or streptozotocin (STZ) injection. MSO, missense oligonucleotides (100 μg). PBS, phosphate buffered saline 0.01 M. (a and b) Levels of Ccl2 mRNA (a) and CCL2 protein (b) in the ipsilateral lumbar 3/4 DRGs on day 35 after CCI or sham surgery in mice with i.t. injection of ASOs, MSOs, or PBS on day 7 post-CCI or sham surgery. n=3 repeats (6 mice)/group/assay. ∗P<0.05, ∗∗P<0.01, by two-way anova followed by post hoc Tukey test. (c and d) Levels of Ccl2 mRNA (c) and CCL2 protein (d) in the ipsilateral lumbar 4 DRG on day 35 after SNL or sham surgery in mice with i.t. injection of ASOs or PBS on day 7 post-SNL or sham surgery. n=3 repeats (12 mice)/group/assay. ∗P<0.05, ∗∗P<0.01, by two-way anova followed by post hoc Tukey test. (e and f) Levels of Ccl2 mRNA (E) and CCL2 protein (f) in the unilateral lumbar 3/4 DRGs on day 35 after the first PTX or vehicle 1 (Veh1) injection in mice with i.t. injection of ASOs or PBS on day 7 after the first PTX or vehicle 1 injection. n=3 repeats (6 mice)/group/assay. ∗∗P<0.01, by two-way anova followed by post hoc Tukey test. (g and h) Levels of Ccl2 mRNA (g) and CCL2 protein (h) in the unilateral lumbar 3/4 DRGs on day 35 after STZ or vehicle 2 (Veh2) injection in mice with i.t. injection of ASOs or PBS on day 14 after STZ or vehicle 2 injection. n=3 repeats (6 mice)/group/assay. ∗P<0.05, ∗∗P<0.01, by two-way anova followed by post hoc Tukey test. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.