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. 2022 Nov 24;130(2):202–216. doi: 10.1016/j.bja.2022.09.027

Fig 6.

Fig 6

Intrathecal (i.t.) administration of nerve injury-specific long non-coding RNA (NIS-lncRNA) antisense oligonucleotides (ASOs, 100 μg) did not produce toxicity in dorsal root ganglion (DRG) and spinal cord. MSO, missense oligonucleotides (100 μg). PBS, phosphate buffered saline 0.01 M. (a–d) Cresyl violet staining in the L4 DRGs and L4 spinal cord of normal mice 30 days after i.t. injection of ASOs, MSOs, or PBS. Representative photograph showing cresyl violet stained cells in lumbar 4 DRG (a) and lumbar 4 spinal cord (c). Quantifying average number of cresyl violet-stained cells in lumbar 4 DRG (b) and in laminae I-II, III-IV, V-VI, and VII-IX of L4 spinal cord (d). n=3 mice/group. One-way anova followed by post hoc Tukey test. (f–h) Representative photograph showing TUNEL-positive cells in the thymus (f), lumbar 4 DRG (g), and lumbar 4 spinal cord (h) of naive mice 30 days after i.t. injection of ASOs, MSOs, or PBS. Thymus was used as a positive control. Scale bars: 100 μm for DRG and thymus and 200 μm for spinal cord.